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    Evaluation of anticancer effects of carboplatin-gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
    (2022) Danışman Kalındemirtaş, Ferdane; Afşin Kariper, İshak; Erdemir, Gökçe; Sert, Esra; Erdem Kuruca, Serap
    Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite efective in anticancer treatments, their use in a wide spectrum and efective treatment possibilities are limited due to their systemic side efects and drug resistance development. In recent years, studies have focused on increasing the therapeutic efcacy of platinum-based drugs with drug delivery systems. Gelatin, a protein, obtained by the hydrolysis of collagen, is a biocompatible and biodegradable material that can be used in nano drug delivery systems. In this study, CP-loaded gelatin-based NPs (CP-NPs) were exposed to IR light in diferent temperatures at 30, 35, 40, 45, and 50 °C and characterized by FESEM-EDX, FTIR, UV–Vis, DLS. Accordingly, we synthesized gelatin-based CP-NPs of diferent sizes between 10–290 nm by exposure to IR. We found that CP-NPs-50, 16 nm nano-sized, obtained at 50 °C had the most cytotoxicity and was 2.2 times more efective than the free drug in HCT 116 colon cancer cells. Moreover, we showed that the cytotoxicity of CP-NPs-50 in normal HUVEC cells was lower. Additionally, we demonstrated that CP-NPs enhanced apoptotic activity while not developing MDR1-related resistance in colon cancer cells. In this study, for the frst time drug loaded gelatin-based nanoparticles were synthesized in diferent sizes with a newly self-assembly method by exposing them to infrared light at diferent temperatures and their anticancer efects were evaluated subsequently.