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Öğe Frost Tolerances of Turkish Olive (Olea europaea L.) Cultivars(Univ Namik Kemal, 2023) Mete, Nurengin; Gulen, Hatice; Cetin, Oznur; Hakan, Mehmet; Guloglu, Ugur; Kaya, Hulya; Ulucay, NurcanThe motherland of the olive is considered as east of the Mediterranean Basin. Turkey is partly located within this basin having a rich source of biodiversity. Olive growing in Turkey is carried out in the Aegean, Marmara, Mediterranean, Southeastern Anatolia and Black Sea Regions. Climatic factors determine the cultivation limits of olive which is not very selective in terms of soil demand. In regions where olives are grown, the Mediterranean climate prevails. Winters are warm and rainy in the region, while summers are hot and dry. On the other hand, severe cold damage in some years can cause significant damage especially in the inner parts of Aegean, Marmara and Black Sea Regions. As a matter of fact, it is known that 31.8% of olive plantations in Turkey are exposed to occasional frost damage. This situation has revealed the necessity of determining the varieties with high frost tolerance and determining the suitable varieties for the regions where cold damage may occur. In the study, 40 registered olive cultivars in Kemalpasa Olive Germplasm Bank in Bornova Olive Research Institute, in Izmir were screened in terms of frost tolerance. To evaluate the frost tolerance (LT50) of cultivars, as estimated by ion leakage (electrical conductivity), leaf samples taken in six different periods over two years were exposed to temperatures at +4 degrees C (control),-2 degrees C,-5 degrees C,-8 degrees C,-11 degrees C,-14 degrees C,-17 degrees C and-20 degrees C. In conclusion; it has been determined that frost tolerance of olive varieties revealed significant variability both genetically and seasonally. Butko, Memeli, Otur, Gemlik, Sinop No 5, Yun celebi, Kara Yaprak, Sati and Sari Ulak were determined as cultivars that were more tolerant of frost. Sinop No 1, Marantelli, Ayvalik, Gorvele, cakir, Samsun Tuzlamalik, Erkence, Saurani, Esek Zeytini (Tekirdag), Kan celebi, Izmir Sofralik, cilli, Samsun Yaglik, Domat, Esek Zeytini (odemis), Sacakli Otur, Sinop No 4, Memecik, Nizip Yaglik, Tekirdag cizmelik and Patos were found to be moderately tolerant cultivars while Edincik, Sinop No 6, cekiste, Mavi, Kiraz, Kilis Yaglik, celebi (Iznik), Trabzon Yaglik, Uslu and Girit olive cultivars were grouped as cultivars having low tolerance to frost. In addition, data showed that cold acclimation in the olive is quite important, and exposure to low temperatures for a certain period of time has significantly increased the frost tolerance. However, this condition was not stable and could rapidly become reversed when temperature reached to the point at which adapdation did not ensure.Öğe Mapping of QTLs associated with frost tolerance in olive (Olea europaea L.)(TUBITAK, 2025) Çetin, Öznur; Mete, Nurengin; Ölmez, Handan; Gülen, HaticeThe olive (Olea europaea L.) is widely distributed due to its high adaptability to different environmental conditions. However, in recent years, sudden extreme temperature decreases and frost damage have caused significant yield and quality losses in some regions. This situation has led to the development of varieties with high frost tolerance, suitable for regions where cold damage may occur. It takes many years to develop varieties using classical breeding methods in olives. Therefore, early selection should be made using biotechnological methods. Markers about any trait provide a great advantage, especially in plant species with a very long juvenile sterility period, as they allow for a selection of nonpurpose plants in the early stage of the breeding process. The elucidation of the molecular mechanism associated with frost stress is important in laying the groundwork for breeding studies. To determine the locations of genes controlling frost tolerance in olives on chromosomes, linkage Quantitative Trait Loci (QTL) and association mapping studies were performed on leaf tissues of Memecik x Uslu hybrids (104 genotypes) subjected to frost tests under controlled conditions in the laboratory. Twenty-three linkage groups (LG) exactly matching the haploid chromosome number (n = 23) of the olive were obtained for the maternal parent Memecik and 26 LG for the paternal parent Uslu. A total of 4377 markers were mapped for the Memecik cultivar and 4664 markers for Uslu. The total number of markers mapped for both parents was 9041. This study investigated candidate QTLs associated with frost tolerance in the olive.