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    Mapping of QTLs associated with frost tolerance in olive (Olea europaea L.)
    (TUBITAK, 2025) Çetin, Öznur; Mete, Nurengin; Ölmez, Handan; Gülen, Hatice
    The olive (Olea europaea L.) is widely distributed due to its high adaptability to different environmental conditions. However, in recent years, sudden extreme temperature decreases and frost damage have caused significant yield and quality losses in some regions. This situation has led to the development of varieties with high frost tolerance, suitable for regions where cold damage may occur. It takes many years to develop varieties using classical breeding methods in olives. Therefore, early selection should be made using biotechnological methods. Markers about any trait provide a great advantage, especially in plant species with a very long juvenile sterility period, as they allow for a selection of nonpurpose plants in the early stage of the breeding process. The elucidation of the molecular mechanism associated with frost stress is important in laying the groundwork for breeding studies. To determine the locations of genes controlling frost tolerance in olives on chromosomes, linkage Quantitative Trait Loci (QTL) and association mapping studies were performed on leaf tissues of Memecik x Uslu hybrids (104 genotypes) subjected to frost tests under controlled conditions in the laboratory. Twenty-three linkage groups (LG) exactly matching the haploid chromosome number (n = 23) of the olive were obtained for the maternal parent Memecik and 26 LG for the paternal parent Uslu. A total of 4377 markers were mapped for the Memecik cultivar and 4664 markers for Uslu. The total number of markers mapped for both parents was 9041. This study investigated candidate QTLs associated with frost tolerance in the olive.
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    Molecularly imprinted polymer nanoparticles for the recognition of ellagic acid
    (WILEY, 2022) Yücel, Necla; Gülen, Hatice; Hatır, Pınar Çakır
    Ellagic acid (EA) is one of the bioactive polyphenolic flavonoids found in many fruits. Over the years, various extraction methods have been developed to isolate pure EA from plant samples. These methods, however, have some disadvantages, such as low specificity and selectivity, not easy incorporation into industrial processes, and low-purification efficiency. The present study proposes an approach based on the molecular imprinting technique. We describe the nanoscale production of EA imprinted polymers for the first time. We develop a straightforward, efficient, and environmentally friendly precipitation polymerization method to synthesize molecularly imprinted polymer nanoparticles (nanoMIPs). To increase the affinity of nanoMIPs, we explore molecular interactions between EA and functional monomers before polymerization. The nanoMIPs are successfully synthesized with an excellent yield of 94.5%. The maximum binding capacity is 66.6 and 45.6 mu mol/g for EA imprinted and nonimprinted polymers, respectively. Furthermore, in the presence of quercetin, an EA analog, and other compounds in the pomegranate peel extract solution, nanoMIPs selectively retain EA. NanoMIPs will be a valuable analytical tool for separation and sensing applications, especially dispersive solid-phase extraction.