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Öğe Clinical comprehensive and experimental assessment of the radioprotective effect of Annona muricata leaf extract to prevent cellular damage in the ileum tissue(De Gruyter Open Ltd, 2022) Elmas, Özlem; Şahin, Havva Hande Keser; Keskin, Emrah; Güven, Berrak; Uslu Erdemir, Rabiye; Almisned, Ghada; Zakaly, Hesham M. H.; Ene, Antoaneta; Tekin, Hüseyin OzanWe report the radioprotective attitude of Annona muricata (AM) leaf extract as antioxidant material to prevent cellular damage in the ileum tissue. The protective effects of an ethyl acetate extract of AM leaves are comprehensively investigated against radiation-induced ileal damage in numerous rats. Thirty-two adult female rats were separated into 4 groups (3 intervention groups and 1 control) as follows: controls received 0.01 mL/kg distilled water, the AM group received 300 mg/kg AM leaf extract, the ionizing radiation (IR) group received a single dose of whole body radiation (8.3 Gy) after 0.01 mL/kg saline treatment, and the AM + IR group received 300 mg/kg AM leaf extract treatment and were subjected to whole body radiation (8.3 Gy) 1 h after the last gavage. All treatments are administered by oral gavage once a day for 9 days. At the end of the experiment, biochemical total oxidant status (TOS, interleukin-6, and caspase) and histological examinations are performed on blood samples as well as ileum tissue. TOS levels are found to be significantly high in rats, which received irradiation, and those in the AM group when compared to controls. These findings suggest that AM has radioprotective effects on ileum tissue, likely because of its antioxidative properties. The findings of this research may contribute to the minimizing of major side effects induced by excessive radiation exposure in patients undergoing radiotherapy and may serve as a significant impetus for further assessments. However, future studies are highly recommended to confirm safety and to determine extraction technique and dosage before human use can be considered.Öğe Comparative effect of ostrich, olive, and omega-3 oils in the prevention of liver damage due to ionizing radiation in rats(Elsevier, 2025) Elmas, Özlem; Şahin, Havva Hande Keser; Güven, Berrak; Almisned, Ghada; Zakaly, Hesham M. H.; Tekin, Hüseyin Ozan; Ene, AntoanetaThe objective of this study was to assess the potential advantageous impact of ostrich oil, olive oil, and omega-3 oil in mitigating the oxidative stress and hepatic tissue damage induced by ionizing radiation in rats. A total of sixty-four female Wistar albino rats were categorized into eight distinct groups, namely: (1) control, (2) irradiation, (3) ostrich oil, (4) ostrich oil combined with irradiation, (5) olive oil, (6) olive oil combined with irradiation, (7) omega-3, and (8) omega-3 combined with irradiation. Omega-3 oil treatment resulted in decreased levels of tissue total oxidant status (TOS) in both healthy and irradiated animals. The study revealed that the rats subjected to radiation and administered with ostrich oil and omega-3 exhibited the lowest levels of tissue total antioxidant status (TAS). The group that was exposed to radiation exhibited significant levels of lymphocyte infiltration, 70% of the samples displaying this characteristic. Additionally, 40% of the samples exhibited ballooning degeneration at the most severe levels, while 60% displayed necrotic changes at a severe level. The animals that were administered omega-3 oil exhibited the least amount of lymphocyte infiltration, ballooning degeneration, and necrotic changes among the groups that were exposed to radiation. The administration of ostrich oil, olive oil, and omega-3 oil at a concentration of 1 mL/kg before exposure to radiation resulted in a reduction of TOS and the restoration of liver morphology. The results of the study indicate that the administration of olive oil to animals resulted in the highest levels of antioxidant activity in irradiated rats. Additionally, rats that received omega-3 oil exhibited the lowest levels of lymphocyte infiltration, ballooning degeneration, and necrosis in liver tissue sections.Öğe A focusing study on radioprotective and antioxidant effects of Annona muricata leaf extract in the circulation and liver tissue: clinical and experimental studies(De Gruyter Open Ltd, 2022) Elmas, Özlem; Şahin, Havva Hande Keser; Güven, Berrak; Abuzaid, Mohamed M.; Elshami, Wiam; Almisned, Ghada; Zakaly, Hesham M. H.; Ene, Antoaneta; Tekin, Hüseyin OzanObjectives: This study investigates the effect of Annona muricata (AM) leaf extract against irradiation-induced damage by the evaluation of hepatic tissue and the levels of oxidative and inflammatory stress in the circulation. Methods: An experimental study with 37 female Wistar albino rats randomized into four groups (controls and three intervention groups) was performed. The first interventional group (group 2) received 300 mg/kg of AM leaf extract by oral gavage once a day for 9 days, group 3 received a single dose of whole-body radiation (8.3 Gy) after a 9-day oral gavage treatment with saline, and the last group received the same irradiation of 8.3 Gy after being treated with 300 mg/kg of AM leaf extract by oral gavage once a day for 9 days. Results: Radiation was found to elevate reactive oxygen species parameters, and AM administration before irradiation was found to decrease total oxidant status (TOS), increase caspase 9, and improve hepatic damage when compared with the group that received only irradiation. Conclusion: The damage caused by irradiation may be ameliorated by the use of the AM extract, which appears to be effective in preventing oxidative stress and inflammatory activity.